Bacteriological tests are performed in food manufacturing factories to detect food poisoning bacteria, but it takes more than 48 hours to obtain results due to the time required for the bacterial incubation process called culturing. Therefore, there remains a demand for rapid testing methods to eliminate food poisoning accidents.
In response to this need, the research team led by Professor Hiroshi Shigi in Osaka Metropolitan University’s Graduate School of Engineering used the optical properties of an organic metal to rapidly and simultaneously treat a food poisoning-inducing drug called enterohemorrhagic Escherichia coli (E. coli O26). Used to identify bacteria. and E. coli O157) and Staphylococcus aureus.
Color signs that alert you to dangerous germs!
The team found for the first time that the organic metal NH generates stronger scattered light than metal nanoparticles of similar size. Since the scattered light of these National Highways remains stable in air for a long time, they are expected to act as stable and highly sensitive labeling material.
Furthermore, it turns out that these NH nanoparticles exhibit different colors of scattered light (white, red and blue) depending on the metallic elements (gold, silver and copper).
The team then introduced antibodies that specifically bind to E. coli O26, E. coli O157, and S. aureus to the organic metal NH and to evaluate the binding properties of antibody-conjugated NH to these NHs of specific bacterial species. use as a label.
As a result, E. coli O26, E. coli O157, and S. aureus was observed under the microscope as white, red and blue scattered light, respectively. In addition, when samples of rotten meat containing different species of bacteria were tested for E. coli O26, E. coli O157, and S. aureus was added, the team was able to use these labels to simultaneously identify each bacterial species added.
This method can identify different types of bacteria by changing the antibodies that are introduced. Furthermore, since it does not require culturing, bacteria can be rapidly detected within an hour, increasing its practicality as a new testing method.
Through this development, we hope to contribute not only to food safety and security, but also to a stable supply of functional foods and a safe and prosperous society in terms of quality control, medical care, drug discovery and public health. also contribute to the construction.